Anti-Bad (phospho S112) antibody (ab1435)

Overview

• Product nameAnti-Bad (phospho S112) antibodySee all Bad primary antibodies ...
• Description
  Rabbit polyclonal to Bad (phospho S112)
• SpecificityThe antibody detects Bad only when phosphorylated at Ser 112.
• Tested applicationsIP, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
• Concentration information loading...
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

Applications

Target

• FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
• Tissue specificityExpressed in a wide variety of tissues.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
• Post-translational
  modificationsPhosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
• Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.

Target information above from: UniProt accession Q92934 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 572 Human
  • Entrez Gene: 12015 Mouse
  • Entrez Gene: 64639 Rat
  • Omim: 603167 Human
  • SwissProt: Q92934 Human
  • SwissProt: Q61337 Mouse
  • SwissProt: O35147 Rat
  • Unigene: 370254 Human

  • Unigene: 4387 Mouse
  • Unigene: 36696 Rat

  see all

• Alternative names
  BAD antibodyBAD_HUMAN antibodyBBC 2 antibody

  BBC2 antibodyBBC6 antibodyBcl 2 Antagonist of Cell Death antibodyBcl 2 Binding Component 6 antibodyBCL X / BCL 2 Binding Protein antibodyBCL X Binding Protein antibodyBcl XL/Bcl 2 Associated Death Promoter antibodyBcl-2-binding component 6 antibodyBcl-2-like protein 8 antibodyBcl-XL/Bcl-2-associated death promoter antibodyBcl2 antagonist of cell death antibodyBCL2 antagonist of cell death protein antibodyBCL2 associated agonist of cell death antibodyBcl2 Associated Death Promoter antibodyBCL2 binding component 6 antibodyBCL2 binding protein antibodyBcl2 Like 8 Protein antibodyBcl2-L-8 antibodyBCL2L8 antibodyBclXL antibodyProapoptotic BH3 Only Protein antibody

  see all

Anti-Bad (phospho S112) antibody images

• 

  Western blot - Bad (phospho S112) antibody (ab1435)
  All lanes : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml
  
  Lane 2 : COS cell lysate. Anti-BAD antibody used as a loading control.
  Lane 3 : COS cell lysate. Anti-BAD antibody used as a loading control.
  Lane 4 : COS cell lysate. Anti-BAD antibody used as a loading control.
  Lane 5 : COS cell lysate. Anti-BAD antibody used as a loading control.
  
  
  Performed under reducing conditions.
  
  Observed band size : 24 kDa (why is the actual band size different from the predicted?)