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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bad (phospho S112) antibody
See all Bad products (27) ...
Description
Rabbit polyclonal to Bad (phospho S112)
Specificity
The antibody detects Bad only when phosphorylated at Ser 112.
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Western blot - Bad (phospho S112) antibody (ab1435)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use a concentration of 5 - 20 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.
Target
Function
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Tissue specificity
Expressed in a wide variety of tissues.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
Cellular localization
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
Target information above from: UniProt accessionQ92934
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAD antibody
- BAD_HUMAN antibody
- BBC 2 antibody
see all
Database links
- Entrez Gene: 572 Human
- Entrez Gene: 12015 Mouse
- Entrez Gene: 64639 Rat
- Omim: 603167 Human
- SwissProt: Q92934 Human
- SwissProt: Q61337 Mouse
- SwissProt: O35147 Rat
- Unigene: 370254 Human
see all
Anti-Bad (phospho S112) antibody images:
Western blot - Bad (phospho S112) antibody (ab1435)
All lanes : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml
Lane 2 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 3 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 4 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 5 : COS cell lysate. Anti-BAD antibody used as a loading control.
Performed under reducing conditions.
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Lane 2: No TPA treatment.
Lane 3: 5 minute TPA treatment.
Lane 4: 30 minute TPA treatment.
Lane 5: 60 minute TPA treatment.
References for Anti-Bad (phospho S112) antibody (ab1435)
ab1435 has not yet been referenced specifically in any publications.
Publishing research using ab1435? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"