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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bag1 antibody
See all Bag1 products (7) ...
Description
Mouse monoclonal to Bag1
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 241-346 of Human Bag1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 31 kDa.
IHC-P: Use a concentration of 3 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
Tissue specificity
Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
Sequence similarities
Contains 1 BAG domain.
Contains 1 ubiquitin-like domain.
Post-translational
modifications
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Cellular localization
Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
Target information above from: UniProt accessionQ99933
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bag 1 antibody
- BAG family molecular chaperone regulator 1 antibody
- BAG-1 antibody
see all
Database links
- Entrez Gene: 573 Human
- Omim: 601497 Human
- SwissProt: Q99933 Human
- Unigene: 377484 Human
Anti-Bag1 antibody images:
IHC-P - Bag1 antibody (ab54817)
Bag1 antibody (ab54817) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot - Bag1 antibody (ab54817)
Bag1 antibody (ab54817) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence-Bag1 antibody(ab54817)
ab54817 at 10 ug/ml staining Bag1 in human Hela cells by Immunocytochemistry / Immunofluorescence.
References for Anti-Bag1 antibody (ab54817)
ab54817 has not yet been referenced specifically in any publications.
Publishing research using ab54817? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"