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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bag3 antibody
See all Bag3 products (7) ...
Description
Rabbit polyclonal to Bag3
Tested applications
IHC-P, WB, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to amino acids 276-294 (REGSPARSSTPLHSPSPIR) of human BAG3.
REGSPARSST PLHSPSPIR
Positive control
Muscle, muscle, PANC-1 pancreatic cell line (ATCC-CRL 1469).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/1000 - 1/5000.IHC-P: 1/1000 - 1/5000.
WB: 1/1000 - 1/2000.Predicted molecular weight: 62 kDa.
IHC-Fr: Use at an assay dependent dilution.
IP
IP: 1/50 - 1/200.IP: 1/50 - 1/200.
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains.
Target information above from: UniProt accessionO95817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Entrez Gene: 293524 Rat
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 15259 Human
- Unigene: 523309 Human
see all
Anti-Bag3 antibody images:
Western blot - Bag3 antibody (ab47107)
All lanes : Anti-Bag3 antibody (ab47107) at 1/2000 dilution
Lane 1 : T3M4
Lane 2 : ASPC-1
Lane 3 : CAPAN-1
Lane 4 : PANC-1
Lane 5 : MIA-PaCa-2
Predicted band size : 62 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
Western blot analysis of BAG3 in human pancreatic cencer cell lines.
Western blot - Bag3 antibody (ab47107)
Predicted band size : 62 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
CAPAN-1 human pancreatic cancer cells were used for all lanes. Cells were heat shocked at 45 degrees for 10 min and total proteins were extracted following incubation at 37 degrees for the indicated times. Control = no heat shock treatment. Maximal BAG3 protein expression was detected at 6 h post heat shock treatment.
References for Anti-Bag3 antibody (ab47107)
ab47107 has not yet been referenced specifically in any publications.
Publishing research using ab47107? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"