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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab47124? Please let us know so that we can cite the reference in this datasheet
ab47124 has been referenced in 3 publications.
- Zhang X & Qian SB Chaperone-mediated hierarchical control in targeting misfolded proteins to aggresomes. Mol Biol Cell 22:3277-88 (2011). PubMed: 21775628
- Crippa V et al. The small heat shock protein B8 (HspB8) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). Hum Mol Genet 19:3440-56 (2010). WB; Mouse. PubMed: 20570967
- Song S et al. Activation of heat shock factor 1 plays a role in pyrrolidine dithiocarbamate-mediated expression of the co-chaperone BAG3. Int J Biochem Cell Biol 42:1856-63 (2010). WB; Human. PubMed: 20692357
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Bag3 antibody (ab47124)
All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size : 62 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
Tissue lysates, normalized for total protein (20
µg/lane), were from a 4 week old male mouse. BAG3
expression was detected at highest levels in skeletal
(quadriceps and diaphragm) and smooth (heart) muscle
specimens.
Immunohistochemistry (Frozen sections) - Bag3 antibody (ab47124)
Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bag3 antibody (ab47124)
Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Immunocytochemistry/ Immunofluorescence-Bag3 antibody(ab47124)
ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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