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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bag3 antibody
See all Bag3 products (7) ...
Description
Rabbit polyclonal to Bag3
Tested applications
ICC/IF, IHC-P, IHC-Fr, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
Positive control
Muscle, PANC-1 pancreatic cell line (ATCC-CRL 1469)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000 - 1/2000.Predicted molecular weight: 62 kDa.
IP
IP: 1/50 - 1/200.IP: 1/50 - 1/200.
Target
Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains.
Target information above from: UniProt accessionO95817
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
Database links
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 15259 Human
- Unigene: 523309 Human
- Unigene: 28373 Mouse
see all
Anti-Bag3 antibody images:
Western blot - Bag3 antibody (ab47124)
All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size : 62 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
Tissue lysates, normalized for total protein (20
µg/lane), were from a 4 week old male mouse. BAG3
expression was detected at highest levels in skeletal
(quadriceps and diaphragm) and smooth (heart) muscle
specimens.
Immunohistochemistry (Frozen sections) - Bag3 antibody (ab47124)
Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bag3 antibody (ab47124)
Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
Immunocytochemistry/ Immunofluorescence-Bag3 antibody(ab47124)
ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Bag3 antibody (ab47124)
This product has been referenced in:
- Zhang X & Qian SB Chaperone-mediated hierarchical control in targeting misfolded proteins to aggresomes. Mol Biol Cell 22:3277-88 (2011).Read more (PubMed: 21775628) »
- Crippa Vet al. The small heat shock protein B8 (HspB8) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS). Hum Mol Genet 19:3440-56 (2010). WB; Mouse.Read more (PubMed: 20570967) »
See all 3 publications for this product
Publishing research using ab47124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"