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Western blot - Bag3 antibody (ab47124)
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Immunohistochemistry (Frozen sections) - Bag3 antibody (ab47124)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bag3 antibody (ab47124)
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Product Name
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Bag3 antibody
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See all Bag3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bag3
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Immunogen
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A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IP: 1/50 - 1/200.
WB: 1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Muscle, PANC-1 pancreatic cell line (ATCC-CRL 1469)
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45 amino acid BAG domain near the C terminus but differ markedly in their N terminal regions. The protein encoded by this gene contains a WW domain in the N terminal region and a BAG domain in the C terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip repressible manner.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bag3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bag3 antibody images, references, products related to ab47124 and other tools.
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Bag3 antibody images:
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 Western blot - Bag3 antibody (ab47124)
All lanes : Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size : 62 kDa
Observed band size : 86 kDa (why is the actual band size different from the predicted?)
Tissue lysates, normalized for total protein (20
µg/lane), were from a 4 week old male mouse. BAG3
expression was detected at highest levels in skeletal
(quadriceps and diaphragm) and smooth (heart) muscle
specimens.
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Immunohistochemistry (Frozen sections) - Bag3 antibody (ab47124)
Frozen mouse muscle tissue section stained for BAG3
expression using ab47124 at 1/2000. The tissue
section was fixed in 3.8% paraformaldehyde prior to
staining. BAG3 localizes with Z-disk proteins."
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bag3 antibody (ab47124)
Immunohistochemistry of formalin fixed
paraffin embedded human pancreas tissue sections
using ab47124 at 1/2000. A, normal pancreas. B and
C, pancreatic cancer. Hematoxylin-eosin counterstain.
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Search PubMed (MEDLINE) for references to Bag3
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Bag3 antibody - more information
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Customer reviews (feedback) regarding Bag3 antibody |
Customer FAQs regarding Bag3 antibody |
Protocols for Bag3 antibody |
Price and availability of products related to Bag3 antibody |
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Bag3 antibody - related products:
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Bag3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bag3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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