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Anti-Bag5 antibody (ab88100) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size : 51 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Anti-Bag5 antibody (ab88100) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 51 kDa
Observed band size : 51 kDa
All lanes : Anti-Bag5 antibody (ab88100) at 1 µg/ml
Lane 1 : Bag5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Predicted band size : 51 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
ab88100, at 10 µg/ml, staining Bag5 in HeLa cell by Immunofluorescence.
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