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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bag5 antibody
See all Bag5 products (6) ...
Description
Mouse polyclonal to Bag5
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human Bag5 (NP_004864.1).
Positive control
WB: Human spleen tissue lysate; HeLa cell lysate; Bag5 transfected 293T cell lysate ICc/IF: HeLa cell
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 51 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.
Sequence similarities
Contains 5 BAG domains.
Domain
The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.
Target information above from: UniProt accessionQ9UL15
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAG family molecular chaperone regulator 5 antibody
- BAG-5 antibody
- BAG5 antibody
see all
Database links
- Entrez Gene: 9529 Human
- Omim: 603885 Human
- SwissProt: Q9UL15 Human
- Unigene: 5443 Human
Anti-Bag5 antibody images:
Western blot - Bag5 antibody (ab88100)
Anti-Bag5 antibody (ab88100) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size : 51 kDa
Observed band size : 44 kDa (why is the actual band size different from the predicted?)
Western blot - Bag5 antibody (ab88100)
Anti-Bag5 antibody (ab88100) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 51 kDa
Observed band size : 51 kDa
Western blot - Bag5 antibody (ab88100)
All lanes : Anti-Bag5 antibody (ab88100) at 1 µg/ml
Lane 1 : Bag5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Predicted band size : 51 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Bag5 antibody (ab88100)
ab88100, at 10 µg/ml, staining Bag5 in HeLa cell by Immunofluorescence.
References for Anti-Bag5 antibody (ab88100)
ab88100 has not yet been referenced specifically in any publications.
Publishing research using ab88100? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"