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- Secondary antibodies
- Lysates
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Product name
Anti-Bak antibody
See all Bak products (12) ...
Description
Rabbit polyclonal to Bak
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to amino acids 23-38 (with C-terminal Lysine added) of Human Bak.
Positive control
A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IHC-P
Application notes
IHC-P: 1/100 (human colon adenocarcinoma).
WB: 1/2000. Detects a band of approximately 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
Tissue specificity
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization
Mitochondrion membrane.
Target information above from: UniProt accessionQ16611
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis Regulator Bak antibody
- BAK 1 antibody
- BAK antibody
see all
Database links
- Entrez Gene: 578 Human
- Omim: 600516 Human
- SwissProt: Q2YD93 Human
- SwissProt: Q6I9T6 Human
- SwissProt: Q16611 Human
- Unigene: 485139 Human
References for Anti-Bak antibody (ab10430)
ab10430 has not yet been referenced specifically in any publications.
Publishing research using ab10430? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"