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Anti-Band 3 (phospho Y21) antibody [EPR1424] (ab125070)


  • Product nameAnti-Band 3 (phospho Y21) antibody [EPR1424]
    See all Band 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR1424] to Band 3 (phospho Y21)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Band 3.

  • Positive control
    • Human red blood cell lysates, Pervanadate treated.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR1424
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab125070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • Tissue specificityDetected in erythrocytes (at protein level). Erythrocytes.
    • Involvement in diseaseElliptocytosis 4
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
    • Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
    • Post-translational
      Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    • Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • Database links
    • Alternative names
      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Anti-Band 3 (phospho Y21) antibody [EPR1424] images

    • All lanes : Anti-Band 3 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (purified)

      Lane 1 : Untreated RBC
      Lane 2 : RBC treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 101 kDa
      Observed band size : 101 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Band 3 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (unpurified)

      Lane 1 : Human red blood cell lysate, untreated
      Lane 2 : Human red blood cell lysate, Pervanadate treated

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution
      developed using the ECL technique

      Predicted band size : 101 kDa

    References for Anti-Band 3 (phospho Y21) antibody [EPR1424] (ab125070)

    ab125070 has not yet been referenced specifically in any publications.

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    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytopl...

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