Recombinant Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1424] to Band 3/AE 1 (phospho Y21)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424]
See all Band 3/AE 1 primary antibodies -
Description
Rabbit monoclonal [EPR1424] to Band 3/AE 1 (phospho Y21) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human red blood cell lysates, Pervanadate treated.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1424 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
Target
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Function
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. -
Tissue specificity
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871). -
Involvement in disease
Ovalocytosis, Southeast Asian
Spherocytosis 4
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Cryohydrocytosis -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Post-translational
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated. -
Cellular localization
Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney. - Information by UniProt
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Database links
- Entrez Gene: 6521 Human
- Omim: 109270 Human
- SwissProt: P02730 Human
- Unigene: 210751 Human
- Unigene: 443948 Human
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Alternative names
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
see all
Images
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All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (purified)
Lane 1 : Untreated RBC
Lane 2 : RBC treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (unpurified)
Lane 1 : Human red blood cell lysate, untreated
Lane 2 : Human red blood cell lysate, Pervanadate treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125070 has not yet been referenced specifically in any publications.