Recombinant Anti-BarX1 antibody [EPR14120] (ab181851)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14120] to BarX1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BarX1 antibody [EPR14120]
See all BarX1 primary antibodies -
Description
Rabbit monoclonal [EPR14120] to BarX1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14120 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). |
Target
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Relevance
BarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 56033 Human
- Entrez Gene: 364680 Rat
- Omim: 603260 Human
- SwissProt: Q9HBU1 Human
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Alternative names
- BarH like homeobox 1 antibody
- BarX 1 antibody
- BARX homeobox 1 antibody
- Homeobox protein BarH like 1 antibody
Images
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All lanes : Anti-BarX1 antibody [EPR14120] (ab181851) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) nuclear fraction lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) without nuclear fraction lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsDiluting/Blocking buffer and concentration: 5% NFDM /TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181851 has been referenced in 3 publications.
- Yan C et al. An esophageal adenocarcinoma susceptibility locus at 9q22 also confers risk to esophageal squamous cell carcinoma by regulating the function of BARX1. Cancer Lett 421:103-111 (2018). WB . PubMed: 29454095
- Guo S et al. GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1. Cell Death Differ N/A:N/A (2018). PubMed: 29523871
- Lin X et al. AP2a enhanced the osteogenic differentiation of mesenchymal stem cells by inhibiting the formation of YAP/RUNX2 complex and BARX1 transcription. Cell Prolif N/A:e12522 (2018). PubMed: 30443989