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Western blot - Bax antibody [6A7] (ab5714)
![Western blot - Bax antibody [6A7] (ab5714)](/ps/datasheet/images/ab5714_1.jpg)
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Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)
![Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)](/ps/datasheet/images/5/ab5714/Bax-Primary-antibodies-ab5714-2.jpg)
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Product Name
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Bax antibody [6A7]
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See all Bax antibodies (21)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [6A7] to Bax
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Immunogen
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Synthetic peptide conjugated to KLH, corresponding to amino acids 12-24 of Human Bax.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml (see Gardai et al reference).
IP: Use at an assay dependent dilution (see Yethon et al reference).
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 20-22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane (alpha isoform) and Cytoplasmic
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Relevance
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Bcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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6A7
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: Borate-buffered saline, pH 8.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bax antibody [6A7] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bax antibody [6A7] images, references, products related to ab5714 and other tools.
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Bax antibody [6A7] images:
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 Western blot - Bax antibody [6A7] (ab5714)
Bax antibody [6A7] (ab5714) at 1 µg/ml + Human HeLa cells, whole cell lysate
Secondary
Goat anti-mouse Alexa Fluor 680 antibody.
Performed under reducing conditions.
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview submitted on 12 December 2005.
See Abreview
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Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)
ab5714 staining human foreskin fibroblast cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin prior to being blocked in 1% BSA + 2% normal goat serum for 30 mins at 20°C. Samples were incubated with 5 µg/ml primary antibody for 45 mins at 20°C; the diution buffer was 1% BSA, 0.1% saponin, 0.05% NaN3 in PBS. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG, dilution 1/1000, was used as secondary antibody. This image is courtesy of an Abreview submitted by Dr Alwin Scharstuhl
See Abreview
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References for Bax antibody [6A7] (ab5714)
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This product has been used in: (two most recent references)
Scharstuhl A et al. Involvement of VDAC, Bax and ceramides in the efflux of AIF from mitochondria during curcumin-induced apoptosis. PLoS One 4:e6688 (2009). ICC/IF; Human. PubMed: 19693275
Maia V et al. C3G silencing enhances STI-571-induced apoptosis in CML cells through p38 MAPK activation, but it antagonizes STI-571 inhibitory effect on survival. Cell Signal 21:1229-35 (2009). WB; Human. PubMed: 19324082
See all 8 publication references for this product.
If you publish research using ab5714 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Bax
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Bax antibody [6A7] - more information
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Customer reviews (feedback) regarding Bax antibody [6A7] |
Customer FAQs regarding Bax antibody [6A7] |
Protocols for Bax antibody [6A7] |
Price and availability of products related to Bax antibody [6A7] |
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Bax antibody [6A7] - related products:
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Bax antibody [6A7] - other tools:
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Contact Abcam with a Technical Enquiry about Bax antibody [6A7] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Bax antibody [6A7] (ab5714)](/index.html?datasheet=5714#ab5714_1.jpg)
![Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)](/index.html?datasheet=5714#Bax-Primary-antibodies-ab5714-2.jpg)
