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Anti-Bax antibody [6A7] (ab5714)

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Overview

Product name

Anti-Bax antibody [6A7]
See all Bax products (23) ...

Description

Mouse monoclonal [6A7] to Bax

Tested applications

Flow Cyt, ICC/IF, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 12-24 of Human Bax.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: None
Constituents: Borate buffered saline, pH 8

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

6A7

Isotype

IgG1

  • Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714) image (enlarge)

  • Flow Cytometry-Bax antibody [6A7](ab5714)Flow Cytometry-Bax antibody [6A7](ab5714) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab5714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Tissue specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Sequence similarities

Belongs to the Bcl-2 family.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Cellular localization

Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.

Target information above from: UniProt accessionQ07812 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • apoptosis regulator BAX antibody
  • Apoptosis regulator BAX cytoplasmic isoform beta antibody
  • Apoptosis regulator BAX membrane isoform alpha antibody
  • BAX antibody
  • Bax isoform psi antibody
  • BAX protein cytoplasmic isoform delta antibody
  • Bax protein cytoplasmic isoform delta. antibody
  • Bax protein cytoplasmic isoform gamma antibody
  • Bax zeta antibody
  • Bax-protein antibody
  • BAX_HUMAN antibody
  • Bcl-2-like protein 4 antibody
  • BCL2 associated X protein antibody
  • Bcl2-L-4 antibody
  • BCL2L4 antibody
  • OTTHUMP00000218291 antibody
  • OTTHUMP00000218292 antibody
  • OTTHUMP00000218293 antibody
  • OTTHUMP00000218294 antibody
  • OTTHUMP00000218295 antibody
  • OTTHUMP00000218296 antibody
see all

Anti-Bax antibody [6A7] images:

  Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)

Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)

ab5714 staining human foreskin fibroblast cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin prior to being blocked in 1% BSA + 2% normal goat serum for 30 mins at 20°C. Samples were incubated with 5 µg/ml primary antibody for 45 mins at 20°C; the diution buffer was 1% BSA, 0.1% saponin, 0.05% NaN3 in PBS. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG, dilution 1/1000, was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr Alwin Scharstuhl

See Abreview

  Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)

Immunocytochemistry/ Immunofluorescence - Bax antibody [6A7] (ab5714)

ab5714 staining Bax in human PMN cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 2% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution. Counterstained with DAPI (blue).

Image courtesy of an anonymous Abreview.

See Abreview

  Flow Cytometry-Bax antibody [6A7](ab5714)

Flow Cytometry-Bax antibody [6A7](ab5714)

Overlay histogram showing HeLa cells stained with ab5714 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5714, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  Western blot - Anti-Bax antibody [6A7] (ab5714)

Western blot - Anti-Bax antibody [6A7] (ab5714)

Anti-Bax antibody [6A7] (ab5714) at 1/2000 dilution + whole tissue lysate prepared from human islet tissue at 20 µg

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/10000 dilution
developed using the ECL technique

Observed band size : 20 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-Bax antibody [6A7] (ab5714)

This product has been referenced in:

  • Ilkow CSet al. The Rubella Virus Capsid Is an Anti-Apoptotic Protein that Attenuates the Pore-Forming Ability of Bax. PLoS Pathog 7:e1001291 (2011). ICC/IF; Human.Read more (PubMed: 21379337) »
  • Kodama Tet al. BH3-only activator proteins Bid and Bim are dispensable for Bak/Bax-dependent thrombocyte apoptosis induced by Bcl-xL deficiency: molecular requisites for the mitochondrial pathway to apoptosis in platelets. J Biol Chem 286:13905-13 (2011). IP; Mouse.Read more (PubMed: 21367852) »

See all 12 publications for this product

Publishing research using ab5714? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"