Western blot abreview for Anti-Bax [E63] antibody

Excellent
Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (h9c2)
Specification h9c2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%MILK
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW
Dilution 1/4000

Detection

Detection method Licor scan
Bands Specific: around 20 kDa
Exposure 5 minute(s) and 0 second(s)
Username

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Submitted Jul 14 2014