Immunocytochemistry/ Immunofluorescence abreview for Anti-Bax [E63] antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Rat Cell (h9c2)
Specification
h9c2
Permeabilization
Yes - 1% TRITON-X-100
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% GOAT SERUM
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit ALEXA 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300

Additional data

Additional Notes
H9C2 cell treated with 400uM H2O2 2h.
Username

Abcam user community

Verified customer

Submitted Jul 14 2014

Sign up