Western blot abreview for Anti-Bax antibody [E63]

Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell lines)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 5 µg
Specification CHO cell lines
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution 1/2000

Secondary antibody

Dilution 1/5000
Name Non-Abcam antibody was used: anti-IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase


Detection method FemtoMax
Exposure 2 second(s)
Bands Specific: ~19 kDa Non-specific: very weak ~70 kDa

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Submitted Mar 11 2016