Western blot abreview for Anti-Bax antibody [E63]

Excellent
Abreviews
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
5 µg
Specification
CHO cell lines
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
FemtoMax
Exposure
2 second(s)
Bands
Specific: ~19 kDa Non-specific: very weak ~70 kDa
Username

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Submitted Mar 11 2016

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