Non-Abcam antibody was used: Biotinylated goat anti rabbit antibody Host species: Goat Clonality: Polyclonal Conjugation: Biotin
Thank you for having tested this antibody in dog, an untested species. The alignment of the immunogen with the target protein sequence is 100%. This percentage suggests there could be some cross-reactivity. We would like to invite more customers to our AbTrial program for testing ab32503 in dog samples. By participating in our Abtrial program you can use this product without financial risk by simply sharing your results in our AbReview system. The Terms and Conditions of this offer can be found at www.abcam.com/abtrial.
The signal is specific but faint. To optimise the intensity, I recommend the following: (1) use the antibody at the dilution 1/250 instead of 1/500, (2) test different durations of antigen retrieval in Citrate buffer.
Abcam user community
Submitted Sep 01 2016
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