Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bax antibody [E63]

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph6.0
Permeabilization No
Specification Tonsil
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 26°C
Fixative Formaldehyde

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution 1/500

Secondary antibody

Dilution 1/500
Name Non-Abcam antibody was used: Biotinylated goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Abcam response Thank you for having tested this antibody in dog, an untested species. The alignment of the immunogen with the target protein sequence is 100%. This percentage suggests there could be some cross-reactivity. We would like to invite more customers to our AbTrial program for testing ab32503 in dog samples. By participating in our Abtrial program you can use this product without financial risk by simply sharing your results in our AbReview system. The Terms and Conditions of this offer can be found at www.abcam.com/abtrial. The signal is specific but faint. To optimise the intensity, I recommend the following: (1) use the antibody at the dilution 1/250 instead of 1/500, (2) test different durations of antigen retrieval in Citrate buffer.
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Submitted Sep 01 2016