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Anti-Bax antibody (ab10813)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab10813 for help.

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2 questions for ab10813

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Question 1

Tuesday 03-April-2012

I'm looking to stain Bax in mouse ICC/IF but I want to be able to differentiate between mitochondrial and lysosomal membrane localization.

ANSWER:

  

Thank you for your inquiry.


We have two antibodies tested in Bax in ICC/IF as ab10813 and ab62641.


http://www.abcam.com/index.html?datasheet=10813 (or use the following: http://www.abcam.com/index.html?datasheet=10813).


http://www.abcam.com/index.html?datasheet=62641 (or use the following: http://www.abcam.com/index.html?datasheet=62641).


You'll probably have to do a colocalization study for what you're trying to accomplish. Take a look at this article which describes something similar:


http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517437/


They used a Bax antibody and a LAMP2 antibody, like our ab37024.


http://www.abcam.com/index.html?datasheet=37024 (or use the following: http://www.abcam.com/index.html?datasheet=37024).


I've also listed some reagents similar to those used in the paper:


Mounting media with DAPI, ab104140I: http://www.abcam.com/Fluoroshield-Mounting-Medium-With-DAPI-ab104140.html


CytoPainter lysosomal and mitochondrial staining kits:


http://www.abcam.com/CytoPainter-Mitochondrial-Staining-Kit-Green-Fluorescence-ab112143.html


http://www.abcam.com/CytoPainter-Lysosomal-Staining-Kit-Red-Fluorescence-ab112137.html


I hope this information helps. Please contact us with any other questions.

Question 2

Wednesday 11-January-2012

Does this antibody recognize total Bax or activated Bax?

ANSWER:

  

Thank you for your inquiry. This antibody will recognize both native and activated BAX. I hope this information helps. Please let me know if you have any additional questions.

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