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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Bax antibody
See all Bax products (23) ...
Description
Rabbit polyclonal to Bax
Tested applications
IHC-P, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to amino acids 43-61 of Human Bax.
Positive control
Normal Breast Tissue.
General notes
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1000 - 1/2000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Detects a band of approximately 21 kDa.
ICC/IF: 1/200
IP: 1/1000
Target
Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Target information above from: UniProt accessionQ07812
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- apoptosis regulator BAX antibody
- Apoptosis regulator BAX cytoplasmic isoform beta antibody
- Apoptosis regulator BAX membrane isoform alpha antibody
see all
Database links
- Entrez Gene: 581 Human
- Entrez Gene: 12028 Mouse
- Omim: 600040 Human
- SwissProt: Q07812 Human
- SwissProt: Q07813 Mouse
- Unigene: 624291 Human
- Unigene: 19904 Mouse
Anti-Bax antibody images:
Immunoprecipitation - Bax antibody (ab10813)
ab10813 immunoprecipitating Bax. 2mg of human neuroblastoma whole cell lysate was incubated with ab10813 at a 1/1000 dilution for 16 hours and then precipitated with protein A. An HRP polyclonal antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by David MacDonald on 6 February 2006.
Immunocytochemistry/ Immunofluorescence - Bax antibody (ab10813)
ab10813 (1/200) staining Bax in assynchronous Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5%Triton X-100/PBS and counterstained with DAPi in order to highlight the nucleus. Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
References for Anti-Bax antibody (ab10813)
This product has been referenced in:
- Ottewell PDet al. Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor growth in bone. Mol Cancer Ther 8:2821-32 (2009). WB; Mouse.Read more (PubMed: 19789217) »
- Abdulamir ASet al. Severity of asthma: the role of CD25+, CD30+, NF-kappaB, and apoptotic markers. J Investig Allergol Clin Immunol 19:218-24 (2009). ICC/IF; Human.Read more (PubMed: 19610265) »
See all 2 publications for this product
Publishing research using ab10813? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"