Overview

  • Product nameAnti-Bax antibodySee all Bax primary antibodies ...
  • Description
    Rabbit polyclonal to Bax
  • Tested applicationsICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Bax.

  • Positive control
    • Extracts from 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab53154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/5000. PubMed: 22629415
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionAccelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificityExpressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationCytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • apoptosis regulator BAX antibody
    • Apoptosis regulator BAX cytoplasmic isoform beta antibody
    • Apoptosis regulator BAX membrane isoform alpha antibody
    • BAX antibody
    • Bax isoform psi antibody
    • BAX protein cytoplasmic isoform delta antibody
    • Bax protein cytoplasmic isoform delta. antibody
    • Bax protein cytoplasmic isoform gamma antibody
    • Bax zeta antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Anti-Bax antibody images

  • Immunofluorescence analysis of A549 cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • All lanes : Anti-Bax antibody (ab53154) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, untreated
    Lane 2 : Extracts from 293 cells, treated with the immunizing peptide


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.

  • Ab53154 staining human normal gallbladder tissue. Staining is localised to cell membrane and cytoplasm.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
  • ELISA analysis of primary cultured rat cortical neurons, detecting Bax using ab53154.
    Cells were treated with (Tat) or without Tat (control) and either Estradiol, Genistein or Daidzein. Lysates were prepared after 24 hours. A 96-well plate was coated using carbonate coating buffer. 20 µg cell lysate was added to wells and incubated overnight at 4°C. Plates were blocked with 1% BSA for 2 hr at room temperature. Primary antibody (1/5000) was added to sample wells before incubating overnight at 4°C. A goat anti-rabbit ALP-conjugated IgG was used as secondary antibody and phosphatase substrate mixture was added. Absorbance was read at 650nm.

References for Anti-Bax antibody (ab53154)

This product has been referenced in:
  • Adams SM  et al. Soy isoflavones genistein and daidzein exert anti-apoptotic actions via a selective ER-mediated mechanism in neurons following HIV-1 Tat(1-86) exposure. PLoS One 7:e37540 (2012). ELISA ; Rat . Read more (PubMed: 22629415) »
  • Adams SM  et al. ER-ß mediates 17ß-estradiol attenuation of HIV-1 Tat-induced apoptotic signaling. Synapse 64:829-38 (2010). Read more (PubMed: 20340172) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"