Anti-Bax antibody (ab53154)
Key features and details
- Rabbit polyclonal to Bax
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bax antibody
See all Bax primary antibodies -
Description
Rabbit polyclonal to Bax -
Host species
Rabbit -
Specificity
Preliminary customer data suggests that the current lot may not work in rat species, therefore it will be removed as a validated species until new testing is available.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Bax. The immunogen in within aa 1-50.
Database link: Q07812 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/100 - 1/500.
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WB |
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 581 Human
- Entrez Gene: 24887 Rat
- Omim: 600040 Human
- SwissProt: Q07812 Human
- SwissProt: Q63690 Rat
- Unigene: 624291 Human
- Unigene: 10668 Rat
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Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
see all
Images
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All lanes : Anti-Bax antibody (ab53154) at 1/500 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.
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ab53154 staining human normal gallbladder tissue. Staining is localized to cell membrane and cytoplasm.
Left panel: ab53154 at 4 µg/ml. Right panel: Isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with the EDTA pH 9.0. Slides were blocked in 3% H2O2, methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (259)
ab53154 has been referenced in 259 publications.
- Zhang XY et al. Benzophenone-3 breaches mouse Sertoli cell barrier and alters F-actin organization without evoking apoptosis. Environ Toxicol 37:28-40 (2022). PubMed: 34558770
- Pan S et al. SOX4 induces drug resistance of colorectal cancer cells by downregulating CYLD through transcriptional activation of microRNA-17. J Biochem Mol Toxicol 36:e22910 (2022). PubMed: 34927777
- Lu F et al. Downregulation of cathepsin C alleviates endothelial cell dysfunction by suppressing p38 MAPK/NF-κB pathway in preeclampsia. Bioengineered 13:3019-3028 (2022). PubMed: 35037834
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Jin M et al. Circ_0011292 knockdown mitigates progression and drug resistance in PTX-resistant non-small-cell lung cancer cells by regulating miR-433-3p/CHEK1 axis. Thorac Cancer 13:1276-1288 (2022). PubMed: 35348291