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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Bax antibody
See all Bax products (23) ...
Description
Rabbit polyclonal to Bax
Specificity
Reacts specifically with Bax.
Tested applications
IP, IHC-P, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: GGPTSSEQIMKTGALLLQGF, corresponding to N terminal amino acids 11-30 of Human Bax.
GGPTSSEQIM KTGALLLQGF
Positive control
HeLa cell lysate, tonsil
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
WB: 1/1000
IHC-Fr: 1/100
Target
Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Target information above from: UniProt accessionQ07812
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- apoptosis regulator BAX antibody
- Apoptosis regulator BAX cytoplasmic isoform beta antibody
- Apoptosis regulator BAX membrane isoform alpha antibody
see all
Database links
- Entrez Gene: 581 Human
- Entrez Gene: 12028 Mouse
- Entrez Gene: 24887 Rat
- Omim: 600040 Human
- SwissProt: Q07812 Human
- SwissProt: Q07813 Mouse
- SwissProt: Q63690 Rat
- Unigene: 624291 Human
see all
Anti-Bax antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bax antibody (ab7977)
Ab7977 staining Human normal bladder. Staining is localized to cytoplasmic compartment.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Bax antibody (ab7977)
All lanes : Anti-Bax antibody (ab7977) at 1/1000 dilution
Lane 1 : CHO whole cell lysate. Non-transfected.
Lane 2 : CHO whole cell lysate. Cell transfected with Bax-EGFP in pcDNA3.1
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-rabbit IRDye 800 at 1/30000 dilution
Performed under reducing conditions.
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa,25 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Bax fused to EGFP should correspond to approximately 48 kDa as EGFP is 28 kDa and BAX is 21 kDa.
8% gel run under denaturing conditions.Blocked using 3% BSA for 2 hours at 25°C.Detection method: Infrared.Cells were transfected using Lipofectamine 2000 and harvested after ~18 hours.
This image is courtesy of an anonymous abreview.
References for Anti-Bax antibody (ab7977)
This product has been referenced in:
- Ilkow CSet al. The Rubella Virus Capsid Is an Anti-Apoptotic Protein that Attenuates the Pore-Forming Ability of Bax. PLoS Pathog 7:e1001291 (2011). WB; Human.Read more (PubMed: 21379337) »
- Kelsen Set al. Endothelin-A receptor blockade slows the progression of renal injury in experimental renovascular disease. Am J Physiol Renal Physiol 301:F218-25 (2011).Read more (PubMed: 21478482) »
See all 17 publications for this product
Publishing research using ab7977? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"