Anti-Bax (phospho S184) antibody (ab111391)

Overview

• Product nameAnti-Bax (phospho S184) antibodySee all Bax primary antibodies ...
• Description
  Rabbit polyclonal to Bax (phospho S184)
• Specificityab111391 detects endogenous levels of Bax only when phosphorylated at serine 184.
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide derived from Human Bax around the phosphorylation site of Serine 184 (T-A-S^P-L-T).

• Positive controlHuman brain tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS (without Mg2+ and Ca2+)
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab111391 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

Applications

Target

• FunctionAccelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
• Tissue specificityExpressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
• Cellular localizationCytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.

Target information above from: UniProt accession Q07812 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 581 Human
  • Entrez Gene: 12028 Mouse
  • Entrez Gene: 24887 Rat
  • Omim: 600040 Human
  • SwissProt: Q07812 Human
  • SwissProt: Q07813 Mouse
  • SwissProt: Q63690 Rat
  • Unigene: 624291 Human

  • Unigene: 19904 Mouse
  • Unigene: 10668 Rat

  see all

• Alternative names
  Apoptosis regulator BAX antibodyBAX antibodyBax-protein antibody

  BAX_HUMAN antibodyBcl-2-like protein 4 antibodyBCL2 associated X protein antibodyBCL2 associated X protein omega antibodyBcl2-L-4 antibodyBCL2L4 antibodyOTTHUMP00000218291 antibodyOTTHUMP00000218292 antibodyOTTHUMP00000218293 antibodyOTTHUMP00000218294 antibodyOTTHUMP00000218295 antibodyOTTHUMP00000218296 antibody

  see all

Anti-Bax (phospho S184) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bax (phospho S184) antibody (ab111391)
  ab111391 at 1/50 dilution staining Bax (phospho S184) in paraffin-embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with synthesized phosphopeptide.