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Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)

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Overview

  • Product nameBcl-2 Human ELISA Kit (with color giving dyes)See all BCL2 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    0.5 ng/ml
  • Range
    0.5 ng/ml - 32 ng/ml
  • Recovery

    71 %

  • Species reactivity
    Reacts with: Human
  • Product overview

    Bcl-2 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2. ab119506 is for research use only. Not for diagnostic or therapeutic procedures.

    Bcl-2 encoded by a proto-oncogene, is an intracellular membrane associated protein that functions to block programmed cell death. Expression of Bcl-2 has been reported in a wide range of hematopoietic cells, nonneoplastic epithelia and epithelial malignancies. Bcl-2 levels are low or undetectable in endothelial cells. A decrease in Bcl-2 levels leads to cell death by apoptosis.

    Overexpression of Bcl-2 on the other hand protects cells from death, but it is neither able to immortalize normal cells, nor to cause tumorgenic transformation of immortalized cells.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Adhesive Films 4 units
    Assay Buffer Concentrate (20x) 1 x 50ml
    Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 100µl
    Blue-Dye 1 x 0.4ml
    Green-Dye 1 x 0.4ml
    Human Bcl-2 Standard 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Monoclonal anti-human Bcl2-coated microwell plate 1 unit
    Red-Dye 1 x 0.4ml
    Sample Diluent 1 x 12ml
    Stop Solution 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    Substrate Solution 1 x 15ml
    Wash Buffer Concentrate (20x) 1 x 50ml
  • Research Areas
  • FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
  • Tissue specificityExpressed in a variety of tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
  • Post-translational
    modifications    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability.
  • Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.

    • Target information above from: UniProt accession P10415 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Apoptosis regulator Bcl-2B cell CLL/lymphoma 2Bcl2
        BCL2_HUMANPPP1R50Protein phosphatase 1 regulatory subunit 50
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119506 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Bcl-2 Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)
        Sandwich ELISA - Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)
        Representative standard curve using ab119506.

      Protocols

      References for Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)

      ab119506 has not yet been referenced specifically in any publications.

      Publishing research using ab119506 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119506 Submit a Question for ab119506

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

      10
      Votes: Highest First

      I am interested in performing an ELISA with your BCL-2 Human Antibody. Approximately which region of the protein does this antibody recognize? Has the binding been tested to the native protein conformation or denatured protein only?

      While we do not have binding information available, AB119506, BML2 assay, detected Bcl-2 in pathological sera from patients with melanoma. The originating lab also performed protein spike recovery assays.


      Inquiry: Hi! I have a question concerning the BCL-2 ELISA kit ab119506. Could you please tell me, which BCL-2 isoform is detected with the kit (alpha or beta). It would also help me, if you could tell me against which epitopes the antibodies are dire...

      Read More

      Hello!

      This kit does recognizes both the alpha and beta isoform of the Bcl-2 protein.

      Please let me know if you have any further questions.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"