Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)
- Product nameBcl-2 Human ELISA Kit (with color giving dyes)See all BCL2 kits ...
Intra-assay Sample n Mean SD CV% Overall 8.6% Inter-assay Sample n Mean SD CV% Overall 12%
- Tests1 x 96 test
- Sample typeSerum, Plasma, Cell culture extracts
- Assay typeSandwich
- Sensitivity0.5 ng/ml
- Range0.5 ng/ml - 32 ng/ml
- Species reactivityReacts with: Human
- Product overview
Bcl-2 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Bcl-2. ab119506 is for research use only. Not for diagnostic or therapeutic procedures.
Bcl-2 encoded by a proto-oncogene, is an intracellular membrane associated protein that functions to block programmed cell death. Expression of Bcl-2 has been reported in a wide range of hematopoietic cells, nonneoplastic epithelia and epithelial malignancies. Bcl-2 levels are low or undetectable in endothelial cells. A decrease in Bcl-2 levels leads to cell death by apoptosis.
Overexpression of Bcl-2 on the other hand protects cells from death, but it is neither able to immortalize normal cells, nor to cause tumorgenic transformation of immortalized cells.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 50ml Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Bcl-2 Standard 2 vials Lysis Buffer (10x) 1 x 15ml Monoclonal anti-human Bcl2-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.
- Apoptosis regulator Bcl-2B cell CLL/lymphoma 2Bcl2
- BCL2_HUMANPPP1R50Protein phosphatase 1 regulatory subunit 50
Our Abpromise guarantee covers the use of ab119506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Bcl-2 Human ELISA Kit (with color giving dyes) (ab119506)
ab119506 has not yet been referenced specifically in any publications.