Western blot abreview for Anti-Bcl2 antibody [E17]

Poor
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (jurkat)
Loading amount
15 µg
Specification
jurkat
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: donkey anti rabbit IgG
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 70 kDa
Positive control
70kDa
Negative control
blank

Additional data

Additional Notes
This antibody produced no specific 26kDa Bcl-2 band, but did, as found by a previous user a very strong 70kDa non specific band
Username

Dr. Karen Rees-Unwin

Verified customer

Submitted Jul 29 2008

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