Western blot abreview for Anti-Bcl2 antibody [E17]

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (jurkat)
Loading amount 15 µg
Specification jurkat
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution 1/500
Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA

Secondary antibody

Name Non-Abcam antibody was used: donkey anti rabbit IgG
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/10000


Detection method ECL
Exposure 2 hour(s), 0 minute(s) and 0 second(s)
Bands Specific: 0 kDa Non-specific: 70 kDa
Positive control 70kDa
Negative control blank

Additional data

Additional Notes This antibody produced no specific 26kDa Bcl-2 band, but did, as found by a previous user a very strong 70kDa non specific band

Dr. Karen Rees-Unwin

Verified customer

Submitted Jul 29 2008