Western blot abreview for Anti-Bcl2 antibody [E17]

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Application Western blot
Sample Human Cell lysate - whole cell (U937 and PBMC)
Loading amount 4e+006 cells
Specification U937 and PBMC
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step 2% Milk with 1% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Other product details

Dilution 1/500
Incubation time 2 hour(s) and 0 minute(s) · Diluent: 2% milk with 1% BSA

Secondary antibody

Name Non-Abcam antibody was used: AbD Serotec STAR54 Sheep anti-rabbit IgG:HRP
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/10000

Detection

Detection method ECL
Exposure 10 minute(s) and 0 second(s)
Bands Specific: none kDa Non-specific: ~70 kDa
Positive control Jurkat Whole Cell Lysate though no bands were seen in this lane

Additional data

Additional Notes Lane 1 - Jurkat Whole Cell Lysate
Lane 2 - U937 Whole Cell Lysate
Lane 3 - PBMC sample 1 Whole Cell Lysate
Lane 4 - PBMC sample 2 Whole Cell Lysate
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Submitted Sep 03 2008