Flow Cytometry abreview for Anti-Bcl2 antibody [E17]

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Abreviews
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Application Flow Cytometry
Sample Human Cell (U937)
Specification U937
Fixation Leucoperm Reagent A
Permeabilization Yes - Leucoperm Reagent B
Gating Strategy Live Cells

Other product details

Dilution 1/200
Incubation time 30 minute(s) · Diluent: PBS

Secondary antibody

Name Non-Abcam antibody was used: AbD Serotec STAR34B Sheep anti-Rabbit IgG:FITC
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution 1/50

Additional data

Additional Notes Rabbit Serum diluted 1/10000 was used as a negative control (shown in purple, filled) Bcl-2 Ab is shown in pink.
Abcam response Thank you for your honest and thoughtful feedback. I would recommend altering the fixation/blocking conditions, as it would appear the antibody isn't even penetrating the cell membrane. If these suggestions do not improve your results, I'd be happy to discuss issuing a free-of-charge replacement antibody.
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Submitted Sep 09 2008