Flow Cytometry abreview for Anti-Bcl2 antibody [E17]

Poor
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (U937)
Specification
U937
Fixation
Leucoperm Reagent A
Permeabilization
Yes - Leucoperm Reagent B
Gating Strategy
Live Cells

Other product details

Dilution
1/200
Incubation time
30 minute(s) · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: AbD Serotec STAR34B Sheep anti-Rabbit IgG:FITC
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/50

Additional data

Additional Notes
Rabbit Serum diluted 1/10000 was used as a negative control (shown in purple, filled) Bcl-2 Ab is shown in pink.
Abcam response
Thank you for your honest and thoughtful feedback. I would recommend altering the fixation/blocking conditions, as it would appear the antibody isn't even penetrating the cell membrane. If these suggestions do not improve your results, I'd be happy to discuss issuing a free-of-charge replacement antibody.
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Submitted Sep 09 2008

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