Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
SampleHuman Cell (U937)
FixationLeucoperm Reagent A
PermeabilizationYes - Leucoperm Reagent B
Gating StrategyLive Cells
Other product details
Incubation time30 minute(s) · Diluent: PBS
NameNon-Abcam antibody was used: AbD Serotec STAR34B Sheep anti-Rabbit IgG:FITC Host species: Sheep Clonality: Polyclonal Conjugation: Fluorescein
Additional NotesRabbit Serum diluted 1/10000 was used as a negative control (shown in purple, filled) Bcl-2 Ab is shown in pink.
Abcam responseThank you for your honest and thoughtful feedback. I would recommend altering the fixation/blocking conditions, as it would appear the antibody isn't even penetrating the cell membrane. If these suggestions do not improve your results, I'd be happy to discuss issuing a free-of-charge replacement antibody.