Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell (U937)
Leucoperm Reagent A
Yes - Leucoperm Reagent B
Other product details
30 minute(s) · Diluent: PBS
Non-Abcam antibody was used: AbD Serotec STAR34B Sheep anti-Rabbit IgG:FITC Host species: Sheep Clonality: Polyclonal Conjugation: Fluorescein
Rabbit Serum diluted 1/10000 was used as a negative control (shown in purple, filled) Bcl-2 Ab is shown in pink.
Thank you for your honest and thoughtful feedback. I would recommend altering the fixation/blocking conditions, as it would appear the antibody isn't even penetrating the cell membrane. If these suggestions do not improve your results, I'd be happy to discuss issuing a free-of-charge replacement antibody.
Abcam user community
Submitted Sep 09 2008
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