Western blot abreview for Anti-Bcl2 antibody [E17]

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat T-cells)
Loading amount 25 µg
Specification Jurkat T-cells
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution 1/1000
Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BLOTTO

Secondary antibody

Name Non-Abcam antibody was used: anti-Rabbit HRP
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/5000

Detection

Detection method ECL+
Exposure 2 minute(s) and 0 second(s)
Bands Specific: ~28 kDa Non-specific: ~35 kDa
Username

Dr. Jonathan Rud

Verified customer

Submitted Feb 11 2010