Western blot abreview for Anti-Bcl-2 antibody [E17]

Poor
Abreviews
Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (MCF-7, MDA-MB-231)
Specification MCF-7, MDA-MB-231
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS
Dilution 1/5000

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/2000

Detection

Detection method ECL
Bands Specific: N/A kDa Non-specific: ~60 kDa
Exposure 2 minute(s) and 0 second(s)
Positive control MCF-7, MDA-MB-231.

Additional data

Abcam response Thank you for sharing this data with us. ab32124 has previously been shown to work in western blot on Jurkat cells (please see image on datasheet). However, this data shows that the current batch of ab32124 is not working, under these experimental conditions. We would encourage customers experiencing problems with this, or any other abcam product, to contact our technical support team on technical@abcam.com.
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Submitted Aug 22 2014