Western blot abreview for Anti-Bcl-2 antibody [E17]

Poor
Abreviews
Application
Western blot
Loading amount
50000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (MCF-7, MDA-MB-231)
Specification
MCF-7, MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: N/A kDa Non-specific: ~60 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
MCF-7, MDA-MB-231.

Additional data

Abcam response
Thank you for sharing this data with us. ab32124 has previously been shown to work in western blot on Jurkat cells (please see image on datasheet). However, this data shows that the current batch of ab32124 is not working, under these experimental conditions. We would encourage customers experiencing problems with this, or any other abcam product, to contact our technical support team on technical@abcam.com.
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Verified customer

Submitted Aug 22 2014

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