Non-Abcam antibody was used: goat anti-rabbit IgG PE Host species: Goat Clonality: Polyclonal Conjugation: Phycoerythrin
Purple plot represents isotype control, green plot represent Bcl-2 in MCF-7 cells. This antibody did work well in flow, however I am concerned that this might not be recognising Bcl-2, because it detected an ~60kDa protein by western blot.
Thank you for sharing this data with us. ab32124 has previously been tested on Jurkat cells in flow cytometry (please see image on datasheet). However, we are concerned that your western blot data shows a ~60kDa band, which is not specific to Bcl-2. Abcam would like to hear from other customers currently using this product.
Abcam user community
Submitted Sep 02 2014
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