Immunocytochemistry/ Immunofluorescence abreview for Anti-Bcl-2 antibody [E17]

Inconclusive
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (MCF-7)
Specification
MCF-7
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum+0.1% BSA+PBS+0.1% Triton X
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
I'm not sure whether this is the correct staining pattern for Bcl-2. I saw a band at ~60kDa with this antibody by western blot, so this nuclear staining could be due to a non-specific protein.
Abcam response
Thank you for sharing your data with us. ab32124 has previously been tested in ICC/IF on Mcf7 cells (please see datasheet) and we saw cytoplasmic staining. Abcam would welcome feedback from other customers currently using this product.
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Submitted Sep 16 2014

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