I'm not sure whether this is the correct staining pattern for Bcl-2. I saw a band at ~60kDa with this antibody by western blot, so this nuclear staining could be due to a non-specific protein.
Thank you for sharing your data with us. ab32124 has previously been tested in ICC/IF on Mcf7 cells (please see datasheet) and we saw cytoplasmic staining. Abcam would welcome feedback from other customers currently using this product.
Abcam user community
Submitted Sep 16 2014
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