Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl-2 antibody [E17]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (DLBCL U2932 cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
DLBCL U2932 cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS
Dilution
1/200

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Username

Abcam user community

Verified customer

Submitted Dec 15 2015

Sign up