PVDF Membrane Used
Total Cell Lysate prepared as described in product documentation, using COmplete Protease Inhibitor cocktail (Roche).
I'm sorry you are experiencing problems with this antibody. You may want to increase primary antibody incubation time to overnight at 4C. Only the initial block and secondary incubation should be performed in 5% nonfat dry milk; if diluting the primary antibody with a blocking reagent, use 5% BSA.
Mr. John Kelly
Submitted Dec 28 2007
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