Bcl10 antibody [151] (ab10432)

Product Name 

Bcl10 antibody [151]

Product type 

Primary antibodies

Description 

Mouse monoclonal [151] to Bcl10

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Epitope 

The epitope recognized by the antibody resides within amino acids 122-168 of human Bcl 10 molecule.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 2 µg/ml. Detects bands of approximately 20, 32 and 37 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Raji whole cell lysate.

Cellular localization 

Cytoplasmic. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells.

Research areas 

Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

Bcl10 / CIPER/ CLAP / mE10 is expressed in many human and murine tissues. It contains a CARD (caspase recruiting domain) domain and is involved in activation of caspase9 to induce apoptosis. It is a cellular homolog of equine herpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF kappaB. Mutations in Bcl10 are observed in many B and T cell lymphomas implicating its role in pathogenesis of human cancer.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

151

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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