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Anti-Bcl10 antibody [4F8E8H8] (ab36585)

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Overview

Product name

Anti-Bcl10 antibody [4F8E8H8]
See all Bcl10 products (16) ...

Description

Mouse monoclonal [4F8E8H8] to Bcl10

Tested applications

ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).

Positive control

MCF-7 cell extract. Human breast and liver carcinoma tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

4F8E8H8

Isotype

IgG1

Research areas

Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585) image (enlarge)

  • Western blot - Bcl10 antibody [4F8E8H8] (ab36585)Western blot - Bcl10 antibody [4F8E8H8] (ab36585) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • 2 Images

    IHC-P

     IHC-P: 1/100 - 1/500.

    IHC-P: 1/100 - 1/500.

  • IHC-Fr

     IHC-Fr: 1/200 - 1/1000.

    IHC-Fr: 1/200 - 1/1000.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/2000.Detec...Read more →

    WB: 1/500 - 1/2000.Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

  • 1 Image

    ICC/IF

     ICC/IF: 1/200 - 1/1000.

    ICC/IF: 1/200 - 1/1000.

Target

Function

Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.

Tissue specificity

Ubiquitous.

Involvement in disease

Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer.

Sequence similarities

Contains 1 CARD domain.

Post-translational
modifications

Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.

Cellular localization

Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.

Target information above from: UniProt accessionO95999 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B cell lymphoma/leukemia10 antibody
  • AI132454 antibody
  • B cell CLL/lymphoma 10 antibody
  • B cell lymphoma/leukemia10 antibody
  • B-cell CLL/lymphoma 10 antibody
  • B-cell leukemia/lymphoma 10 antibody
  • B-cell lymphoma/leukemia 10 antibody
  • Bcl 10 antibody
  • Bcl-10 antibody
  • Bcl10 antibody
  • BCL10_HUMAN antibody
  • c E10 antibody
  • c-E10 antibody
  • C81403 antibody
  • CARD containing apoptotic signaling protein antibody
  • CARD containing molecule enhancing NF kappa B antibody
  • CARD containing molecule enhancing NF kB antibody
  • CARD containing molecule enhancing NFkB antibody
  • CARD containing proapoptotic protein antibody
  • CARD like apoptotic protein antibody
  • CARD-containing molecule enhancing NF-kappa-B antibody
  • CARD-like apoptotic protein antibody
  • CARMEN antibody
  • Caspase recruiting domain containing protein antibody
  • cCARMEN antibody
  • cE 10 antibody
  • cE10 antibody
  • CED 3/ICH 1 prodomain homologous E10 like regulator antibody
  • CED-3/ICH-1 prodomain homologous E10-like regulator antibody
  • CED3/ICH1 prodomain homologous E10 like regulator antibody
  • Cellular E10 antibody
  • Cellular homolog of vCARMEN antibody
  • Cellular-E10 antibody
  • CIPER antibody
  • CLAP antibody
  • hCLAP antibody
  • Mammalian CARD containing adapter molecule E10 antibody
  • Mammalian CARD-containing adapter molecule E10 antibody
  • mE 10 antibody
  • mE10 antibody
  • R-RCD1 antibody
see all

Database links

Anti-Bcl10 antibody [4F8E8H8] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)

Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bcl10 antibody [4F8E8H8] (ab36585)

Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.

  Western blot - Bcl10 antibody [4F8E8H8] (ab36585)

Western blot - Bcl10 antibody [4F8E8H8] (ab36585)

All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution

Lane 1 : Cell lysates prepared from NIH/3T3 cells
Lane 2 : Cell lysates prepared from Hela cells
Lane 3 : Cell lysates prepared from MCF-7 cells
Lane 4 : Cell lysates prepared from Jurkat cells

Lysates/proteins at 100 µg per lane.

Secondary
HRP-conjugated Goat polyclonal to mouse IgG1

Predicted band size : 31 kDa

  Immunocytochemistry/ Immunofluorescence - Bcl10 antibody [4F8E8H8] (ab36585)

Immunocytochemistry/ Immunofluorescence - Bcl10 antibody [4F8E8H8] (ab36585)

ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.

  Flow Cytometry - Bcl10 antibody [4F8E8H8] (ab36585)

Flow Cytometry - Bcl10 antibody [4F8E8H8] (ab36585)

Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).

References for Anti-Bcl10 antibody [4F8E8H8] (ab36585)

ab36585 has not yet been referenced specifically in any publications.

Publishing research using ab36585? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"