![Western blot - Bcl10 antibody [EP606Y] (ab33905) image](#ab33905-1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [EP606Y] (ab33905) image](#ab33905-2.JPG)
![Immunofluorescence - Bcl10 antibody [EP606Y] (ab33905) image](#ab33905-3.JPG)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bcl10 antibody [EP606Y]
See all Bcl10 products (16) ...
Description
Rabbit monoclonal [EP606Y] to Bcl10
Tested applications
IF, WB, IHC-P, ICC, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in the C terminal of human Bcl10
Positive control
Raji cell lysate, Hela cell lysate and human hepatocellular carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
EP606Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: 1/100 - 1/250.
IF: Use at an assay dependent dilution.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer.
Sequence similarities
Contains 1 CARD domain.
Post-translational
modifications
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionO95999
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
Anti-Bcl10 antibody [EP606Y] images:
Western blot - Bcl10 antibody [EP606Y] (ab33905)
![Western blot - Bcl10 antibody [EP606Y] (ab33905)](/ps/datasheet/Images/33/ab33905/ab33905-1.JPG)
Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/5000 dilution + Raji cell lysate
Predicted band size : 31 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [EP606Y] (ab33905)
![Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [EP606Y] (ab33905)](/ps/datasheet/Images/33/ab33905/ab33905-2.JPG)
ab33905, at a 1/100 dilution, staining human hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Immunofluorescence - Bcl10 antibody [EP606Y] (ab33905)
![Immunofluorescence - Bcl10 antibody [EP606Y] (ab33905)](/ps/datasheet/Images/33/ab33905/ab33905-3.JPG)
ab33905, staining Hela cells by Immunofluorescent.
Western blot
Anti-Bcl10 antibody [EP606Y] (ab33905) at 1/1000 dilution + Bcl10 protein (His tag) (ab82241) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-Bcl10 antibody [EP606Y] (ab33905)
ab33905 has not yet been referenced specifically in any publications.
Publishing research using ab33905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"