Anti-Bcl10 antibody [EPR3174] (ab108412)

Overview

• Product nameAnti-Bcl10 antibody [EPR3174]See all Bcl10 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3174] to Bcl10
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues in Human Bcl10.

• Positive controlDaudi and Raji cell lysates; Raji cells
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3174
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • NFkB pathway

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • CARD Family

Applications

Target

• FunctionPromotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
• Tissue specificityUbiquitous.
• Involvement in diseaseNote=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  Note=Defects in BCL10 are involved in various types of cancer.
• Sequence similaritiesContains 1 CARD domain.
• Post-translational
  modificationsPhosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
• Cellular localizationCytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.

Target information above from: UniProt accession O95999 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8915 Human
  • Omim: 603517 Human
  • SwissProt: O95999 Human
  • Unigene: 193516 Human

• Alternative names
  AI132454 antibodyB cell CLL/lymphoma 10 antibodyB cell lymphoma/leukemia10 antibody

  B-cell CLL/lymphoma 10 antibodyB-cell leukemia/lymphoma 10 antibodyB-cell lymphoma/leukemia 10 antibodyBcl 10 antibodyBcl-10 antibodyBcl10 antibodyBCL10_HUMAN antibodyc E10 antibodyc-E10 antibodyC81403 antibodyCARD containing apoptotic signaling protein antibodyCARD containing molecule enhancing NF kappa B antibodyCARD containing molecule enhancing NF kB antibodyCARD containing molecule enhancing NF-kB antibodyCARD containing molecule enhancing NFkB antibodyCARD containing proapoptotic protein antibodyCARD like apoptotic protein antibodyCARD-containing apoptotic signaling protein antibodyCARD-containing molecule enhancing NF-kappa-B antibodyCARD-containing proapoptotic protein antibodyCARD-like apoptotic protein antibodyCARMEN antibodyCaspase recruiting domain containing protein antibodycaspase-recruiting domain-containing protein antibodycCARMEN antibodycE 10 antibodycE10 antibodyCED 3/ICH 1 prodomain homologous E10 like regulator antibodyCED-3/ICH-1 prodomain homologous E10-like regulator antibodyCED3/ICH1 prodomain homologous E10 like regulator antibodyCellular E10 antibodyCellular homolog of vCARMEN antibodyCellular-E10 antibodyCIPER antibodyCLAP antibodyhCLAP antibodyMammalian CARD containing adapter molecule E10 antibodyMammalian CARD-containing adapter molecule E10 antibodymE 10 antibodymE10 antibodyR-RCD1 antibody

  see all

Anti-Bcl10 antibody [EPR3174] images

• 

  Western blot - Bcl10 antibody [EPR3174] (ab108412)
  All lanes : Anti-Bcl10 antibody [EPR3174] (ab108412) at 1/1000 dilution
  
  Lane 1 : Daudi cell lysate
  Lane 2 : Raji cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 26 kDa
  
• 

  Flow Cytometry - Bcl10 antibody [EPR3174] (ab108412)
  Flow cytometric analysis of Bcl10 in permeabilized Raji cells, using ab108412 at a 1/500 dilution (red) or a Rabbit IgG (negative) (green).