![Western blot - Bcl10 antibody [EPR3175] (ab108328) image](#Bcl10-Primary-antibodies-ab108328-4.JPG)
![Immunocytochemistry/ Immunofluorescence - Bcl10 antibody [EPR3175] (ab108328) image](#Bcl10-Primary-antibodies-ab108328-5.JPG)
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Product name
Anti-Bcl10 antibody [EPR3175]
See all Bcl10 products (17) ...
Description
Rabbit monoclonal [EPR3175] to Bcl10
Tested applications
ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in Human Bcl10.
Positive control
Daudi, Raji, HeLa, and HuT-78 cell lysates; HeLa cells
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3175
Isotype
IgG1
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab108328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 - 1/250.
WB: 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
IP
IP: 1/10 - 1/100.IP: 1/10 - 1/100.
Application notes
Is unsuitable for ,Flow Cyt or IHC-P.
Target
Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer.
Sequence similarities
Contains 1 CARD domain.
Post-translational
modifications
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionO95999
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
Anti-Bcl10 antibody [EPR3175] images:
Western blot - Bcl10 antibody [EPR3175] (ab108328)
![Western blot - Bcl10 antibody [EPR3175] (ab108328)](/ps/datasheet/images/108/ab108328/Bcl10-Primary-antibodies-ab108328-4.JPG)
All lanes : Anti-Bcl10 antibody [EPR3175] (ab108328) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 26 kDa
Immunocytochemistry/ Immunofluorescence - Bcl10 antibody [EPR3175] (ab108328)
![Immunocytochemistry/ Immunofluorescence - Bcl10 antibody [EPR3175] (ab108328)](/ps/datasheet/images/108/ab108328/Bcl10-Primary-antibodies-ab108328-5.JPG)
ab108328, at 1/100 dilution, staining Bcl10 in HeLa cells by Immunofluorescence.
References for Anti-Bcl10 antibody [EPR3175] (ab108328)
ab108328 has not yet been referenced specifically in any publications.
Publishing research using ab108328? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"