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Anti-Bcl10 antibody [ep605y] (ab40752)

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1 question for ab40752

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Question 1

Wednesday 12-January-2011

Do the following antibodies recognize an extracellular epitope or do you have to follow an intracellular protocol for flow?

ab238, 80392, 40752, 32041, 76429, 32135, 76311, 10684, 32536, 32360

ANSWER:

  

Thank you for contacting us.

Flow cytometry protocols are recommended below. For most of the antibodies we are not able to provide specific epitope information but it seems most targets are intracellular. Hence not on the membrane and cells need fixation prior to staining.

ab238- intracellular target. Intracellular protocol is recommended for flow cytometry. ab10684 -intracellular target. Intracellular protocol is recommended for flow cytometry. ab32041- intracellular target. Intracellular protocol is recommended for flow cytometry. ab32135- intracellular target. Intracellular protocol is recommended for flow cytometry. ab32536- intracellular target. Intracellular protocol is recommended for flow cytometry. ab32360- intracellular target. Intracellular protocol is recommended for flow cytometry. ab40752- intracellular target. Intracellular protocol is recommended for flow cytometry. ab76311- intracellular target. Intracellular protocol is recommended for flow cytometry. ab76429- intracellular target. Intracellular protocol is recommended for flow cytometry. ab80392- Bcl10 antibody [151] (Phycoerythrin) - Does not need a secondary detection antibody. Intracellular target. Intracellular protocol is recommended for flow cytometry.

Our protocols are found on http://www.abcam.com/protocols I have attached a protocol for intracellular targets from one of our collaborators (this protocol is also inluded under the link)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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