![Western blot - Bcl10 antibody [ep605y] (ab40752) image](#ab40752_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [ep605y] (ab40752) image](#ab40752_2.jpg)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bcl10 antibody [ep605y]
See all Bcl10 products (16) ...
Description
Rabbit monoclonal [ep605y] to Bcl10
Tested applications
WB, IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues near the N-terminal (Human)
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Clonality
Monoclonal
Clone number
ep605y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Nuclear signaling >> NFkB pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> CARD Family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
IHC-P: 1/50
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
IP
IP: 1/30IP: 1/30
Target
Function
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer.
Sequence similarities
Contains 1 CARD domain.
Post-translational
modifications
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
Cellular localization
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accessionO95999
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell lymphoma/leukemia10 antibody
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
see all
Database links
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
Anti-Bcl10 antibody [ep605y] images:
Western blot - Bcl10 antibody [ep605y] (ab40752)
![Western blot - Bcl10 antibody [ep605y] (ab40752)](/ps/datasheet/Images/40/ab40752/ab40752_1.jpg)
Anti-Bcl10 antibody [ep605y] (ab40752) at 1/2000 dilution + Raji cell lysate at 10 µg
Predicted band size : 31 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [ep605y] (ab40752)
![Immunohistochemistry (Paraffin-embedded sections) - Bcl10 antibody [ep605y] (ab40752)](/ps/datasheet/Images/40/ab40752/ab40752_2.jpg)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ab40752 at a dilution of 1/50.
References for Anti-Bcl10 antibody [ep605y] (ab40752)
ab40752 has not yet been referenced specifically in any publications.
Publishing research using ab40752? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"