Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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ab692 has been referenced in 15 publications.
Publishing research using ab692? Please let us know so that we can cite the reference in this datasheet
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Bcl2 was localized distinctly in specific cytoplasmic regions in human epithelium (especially in the lower and outmost layers). This image was kindly supplied as part of the review submitted by Marko Nykanen.
ab692 diluted 1/100 in BSA was incubated with whole cell lysate of human lymphocytes and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation. 1,000,000 cells were present in the lysate.
A modified RIPA buffer was used to lyse cells.
The IP was washed with PBS/Triton.
ab692 was used at a 1/1000 dilution for the Western Blot step.
This image is courtesy of an anonymous Abreview
Overlay histogram showing Jurkat cells stained with ab692 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab692, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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