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Anti-Bcl2 antibody [100/D5] (ab692)

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Overview

Product name

Anti-Bcl2 antibody [100/D5]
See all Bcl2 products (33) ...

Description

Mouse monoclonal [100/D5] to Bcl2

Specificity

This antibody recognises a protein of 25-26kDa, identified as the bcl-2a oncoprotein. It does not cross-react with bcl-x or bax protein.

Tested applications

Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A Synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys), of human bcl-2 protein.

GAAPAPGIFSSQPG-Cys

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Follicular lymphomas or tonsil.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant, PBS, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

100/D5

Myeloma

P3-NS1/1-Ag4-1

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

  • Immunohistochemistry (Frozen sections) - Bcl2 antibody [100/D5] (ab692)Immunohistochemistry (Frozen sections) - Bcl2 antibody [100/D5] (ab692) image (enlarge)

  • Immunoprecipitation - Bcl2 antibody [100/D5] (ab692)Immunoprecipitation - Bcl2 antibody [100/D5] (ab692) image (enlarge)

  • Flow Cytometry-Bcl2 antibody [100/D5](ab692)Flow Cytometry-Bcl2 antibody [100/D5](ab692) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Tissue specificity

Expressed in a variety of tissues.

Involvement in disease

Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

Sequence similarities

Belongs to the Bcl-2 family.

Domain

The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.

Post-translational
modifications

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.

Cellular localization

Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accessionP10415 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Apoptosis regulator Bcl 2 antibody
  • Apoptosis regulator Bcl-2 antibody
  • Apoptosis regulator Bcl2 antibody
  • AW986256 antibody
  • B cell CLL/lymphoma 2 antibody
  • B cell leukemia/lymphoma 2 antibody
  • B cell lymphoma 2 antibody
  • Bcl 2 antibody
  • Bcl-2 antibody
  • Bcl2 antibody
  • BCL2 protein antibody
  • BCL2_HUMAN antibody
  • C430015F12Rik antibody
  • D630044D05Rik antibody
  • D830018M01Rik antibody
  • Leukemia/lymphoma, B-cell, 2 antibody
  • Oncogene B-cell leukemia 2 antibody
see all

Database links

Anti-Bcl2 antibody [100/D5] images:

  Immunohistochemistry (Frozen sections) - Bcl2 antibody [100/D5] (ab692)

Immunohistochemistry (Frozen sections) - Bcl2 antibody [100/D5] (ab692)

Bcl2 was localized distinctly in specific cytoplasmic regions in human epithelium (especially in the lower and outmost layers). This image was kindly supplied as part of the review submitted by Marko Nykanen.

 

 

  Immunoprecipitation - Bcl2 antibody [100/D5] (ab692)

Immunoprecipitation - Bcl2 antibody [100/D5] (ab692)

ab692 diluted 1/100 in BSA was incubated with whole cell lysate of human lymphocytes and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation.  1,000,000 cells were present in the lysate.

A modified RIPA buffer was used to lyse cells.

The IP was washed with PBS/Triton.

ab692 was used at a 1/1000 dilution for the Western Blot step.

This image is courtesy of an anonymous Abreview

See Abreview

  Flow Cytometry-Bcl2 antibody [100/D5](ab692)

Flow Cytometry-Bcl2 antibody [100/D5](ab692)

Overlay histogram showing Jurkat cells stained with ab692 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab692, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Bcl2 antibody [100/D5] (ab692)

This product has been referenced in:

  • Jiao LR  et al. MicroRNAs targeting oncogenes are down-regulated in pancreatic malignant transformation from benign tumors. PLoS One 7:e32068 (2012). WB; Human.Read more (PubMed: 22384141) »
  • Clark SE  et al. Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease. Br J Cancer 104:120-7 (2011). IHC-P; Human.Read more (PubMed: 21139586) »

See all 15 publications for this product

Publishing research using ab692? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"