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ab18624 |
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ab84209 |
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Read our guarantee »Publishing research using ab18210? Please let us know so that we can cite the reference in this datasheet
ab18210 has been referenced in 5 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Bcl2 antibody (ab18210) at 1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible cross reactivity).
ab18210 recognizes a band of 26 kDa corresponding to Bcl2. A non-specific band of ~ 60 kDa is also detected. Both bands are quenched by the addition of the immunizing peptide (ab18624).
ICC/IF image of ab18210 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18210, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
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