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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bcl2 antibody
See all Bcl2 products (30) ...
Description
Rabbit polyclonal to Bcl2
Specificity
Detects a band at 26 kDa in Jurkat cell lysate that represents Bcl2.
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Bcl2.
(Peptide available as ab18624.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration. (PubMed: 21624358)
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 26 kDa.Can be blocked with Bcl2 peptide (ab18624).
Target
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Tissue specificity
Expressed in a variety of tissues.
Involvement in disease
Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
Post-translational
modifications
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.
Cellular localization
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionP10415
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
Database links
- Entrez Gene: 403416 Dog
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Entrez Gene: 24224 Rat
- Omim: 151430 Human
- SwissProt: Q6R755 Dog
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
see all
Anti-Bcl2 antibody images:
Western blot - Bcl2 antibody (ab18210)
All lanes : Anti-Bcl2 antibody (ab18210) at 1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with Bcl2 peptide (ab18624) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible cross reactivity).
ab18210 recognizes a band of 26 kDa corresponding to Bcl2. A non-specific band of ~ 60 kDa is also detected. Both bands are quenched by the addition of the immunizing peptide (ab18624).
Immunocytochemistry/ Immunofluorescence - Bcl2 antibody (ab18210)
ICC/IF image of ab18210 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18210, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
References for Anti-Bcl2 antibody (ab18210)
This product has been referenced in:
- Wu Xet al. Differentiation of the ductal epithelium and smooth muscle in the prostate gland are regulated by the Notch/PTEN-dependent mechanism. Dev Biol 356:337-49 (2011). IHC-P; Mouse.Read more (PubMed: 21624358) »
- Cronin Tet al. The disruption of the rod-derived cone viability gene leads to photoreceptor dysfunction and susceptibility to oxidative stress. Cell Death Differ : (2010). WB; Mouse.Read more (PubMed: 20139892) »
See all 5 publications for this product
Publishing research using ab18210? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"