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Western blot - Bcl2 antibody (ab18210)
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Immunocytochemistry/ Immunofluorescence - Bcl2 antibody (ab18210)
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Product Name
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Bcl2 antibody
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See all Bcl2 antibodies (23)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bcl2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Bcl2.
(Peptide available as ab18624.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Dog
(Due to sequence homology)
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Specificity
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Detects a band at 26 kDa in Jurkat cell lysate that represents Bcl2.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 26 kDa. Can be blocked with Bcl2 peptide (ab18624).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Endoplasmic reticulum; outer mitochondrial membrane; intracellular membrane of the nuclear envelope.
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Research areas
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Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends.
BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bcl2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bcl2 antibody images, references, products related to ab18210 and other tools.
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Bcl2 antibody images:
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 Western blot - Bcl2 antibody (ab18210)
All lanes : Bcl2 antibody (ab18210) at 1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with Bcl2 peptide (ab18624) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa (possible cross reactivity).
ab18210 recognizes a band of 26 kDa corresponding to Bcl2. A non-specific band of ~ 60 kDa is also detected. Both bands are quenched by the addition of the immunizing peptide (ab18624).
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Immunocytochemistry/ Immunofluorescence - Bcl2 antibody (ab18210)
ICC/IF image of ab18210 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with
the antibody (ab18210, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa
Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal
Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was
used to stain the cell nuclei (blue).
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References for Bcl2 antibody (ab18210)
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This product has been used in:
Lagna G et al. BMP-dependent activation of caspase-9 and caspase-8 mediates apoptosis in pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 291:L1059-67 (2006). PubMed: 17030903
If you publish research using ab18210 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Bcl2
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Bcl2 antibody - more information
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Customer reviews (feedback) regarding Bcl2 antibody |
Customer FAQs regarding Bcl2 antibody |
Protocols for Bcl2 antibody |
Price and availability of products related to Bcl2 antibody |
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Bcl2 antibody - related products:
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Bcl2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bcl2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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