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ab7973 has been referenced in 11 publications.
Publishing research using ab7973? Please let us know so that we can cite the reference in this datasheet
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Fixation: 4% paraformaldehyde, paraffin embedding. Before the beginning of the procedure, slides are put in a 200 ml Coplin jar filled with 10 mM citrate buffer and heated in a commercial microwave oven operating at a frequency of 2.45 GHz and 600 W power setting. After two heating cycles of 5 minutes each, slides are allowed to cool at room temperature and thoroughly washed in phosphate buffered saline (PBS) pH 7.4 0.1 M. ABC peroxidase + Nickel intensification. Conterstaining Methyl green
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Sample treated using same protocol described in the above image.
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Sample treated using same protocol described in the above image, except with no countertaining.
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Anti-Bcl2 antibody (ab7973) at 1/500 dilution + 25 ug HeLa whole cell lysate
Secondary
Goat anti rabbit HRP at 1/4000 dilution
This image is an edited version of an image submitted courtesy of an anonymous Abreview on 30 September 2005. We do not have any further information relating to this image.
ICC/IF image of ab7973 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7973, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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