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Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
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Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
(enlarge)
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Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
(enlarge)
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Product Name
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Bcl2 antibody
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See all Bcl2 antibodies (23)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bcl2
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Immunogen
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Synthetic peptide: AGRTGYDNREIVMKYIHY, corresponding to N terminal amino acids 1-18 of Human Bcl2.
BLAST 'AGRTGYDNREIVMKYIHY' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Cow
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Specificity
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Reacts with Bcl-2
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Tested applications
(see key)
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ICC, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-Fr: 1/100.
IP: Use at an assay dependent dilution.
WB: 1/100.
IHC-P: We have data to suggest that this antibody works on paraffin sections, however, we would recommed to use frozen sections and cannot guarantee IHC-P. In successful cases, the following protocol was used:
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Before the beginning of the procedure, slides should be put in a 200 ml Coplin jar filled with 10 mM citrate buffer and heated in a commercial microwave oven operating at a frequency of 2.45 GHz and 600 W power setting. After two heating cycles of 5 minutes each, slides should be allowed to cool at room temperature and thoroughly washed in phosphate buffered saline (PBS) pH 7.4 0.1 M.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: HeLa, HL-60 and Jurkat lysate
IHC: mouse cerebral cortex, cerebellum, hippocampus
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Cellular localization
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Endoplasmic reticulum; outer mitochondrial membrane; intracellular membrane of the nuclear envelope.
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Research areas
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Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends.
BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bcl2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bcl2 antibody images, references, products related to ab7973 and other tools.
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Bcl2 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
IHC of mouse cerebral cortex
Fixation: 4% paraformaldehyde,
paraffin embedding.
Before the beginning of the procedure, slides are put in a 200 ml Coplin jar filled with 10 mM citrate buffer and heated in a commercial microwave oven operating at a frequency of 2.45 GHz and 600 W power setting. After two heating cycles of 5 minutes each, slides are allowed to cool at room temperature and thoroughly washed in phosphate buffered saline (PBS) pH 7.4 0.1 M.
ABC peroxidase + Nickel intensification.
Conterstaining Methyl green Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
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Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
IHC of mouse cerebellum
Sample treated using same protocol described in the above image. Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
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Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
IHC of mouse hippocampus
Sample treated using same protocol described in the above image, except with no countertaining. Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
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Western blot - Bcl2 antibody (ab7973)
Bcl2 antibody (ab7973) at 1/500 dilution + 25 ug HeLa whole cell lysate
Secondary
Goat anti rabbit HRP at 1/4000 dilution
This image is an edited version of an image submitted courtesy of an anonymous Abreview on 30 September 2005. We do not have any further information relating to this image.
See Abreview
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Immunocytochemistry/ Immunofluorescence-Bcl2 antibody(ab7973)
ICC/IF image of ab7973 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Bcl2 antibody (ab7973)
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This product has been used in: (two most recent references)
Lepsch LB et al. Cocaine induces cell death and activates the transcription nuclear factor kappa-b in pc12 cells. Mol Brain 2:3 (2009). WB; Human. PubMed: 19183502
Beilke LD et al. Decreased apoptosis during CAR-mediated hepatoprotection against lithocholic acid-induced liver injury in mice. Toxicol Lett 188:38-44 (2009). WB; Mouse. PubMed: 19433268
See all 8 publication references for this product.
If you publish research using ab7973 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Bcl2
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Bcl2 antibody - more information
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Customer reviews (feedback) regarding Bcl2 antibody |
Customer FAQs regarding Bcl2 antibody |
Protocols for Bcl2 antibody |
Price and availability of products related to Bcl2 antibody |
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Bcl2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bcl2 antibody |
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