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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Anti-Bcl2 antibody
See all Bcl2 products (30) ...
Description
Rabbit polyclonal to Bcl2
Specificity
Reacts with Bcl-2
Tested applications
IHC-P, IHC-Fr, ICC, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Synthetic peptide: AGRTGYDNREIVMKYIHY, corresponding to N terminal amino acids 1-18 of Human Bcl2.
AGRTGYDNRE IVMKYIHY
Positive control
WB: HeLa, HL-60 and Jurkat lysate IHC: mouse cerebral cortex, cerebellum, hippocampus
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Signal Transduction >> Metabolism >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.(PubMed: 20800980In successful cases, the following protocol was used. Before the beginning of the procedure, slides should be put in a 200 ml Coplin jar filled with 10 mM citrate buffer and heated in a commercial microwave oven operating at a frequency of 2.45 GHz and 600 W power setting. After two heating cycles of 5 minutes each, slides should be allowed to cool at room temperature and thoroughly washed in phosphate buffered saline (PBS) pH 7.4 0.1 M.)
IHC-Fr: 1/100(We have data to suggest that this antibody works on paraffin sections, however, we would recommed to use frozen sections and cannot guarantee IHC-P.)
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100
Target
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Tissue specificity
Expressed in a variety of tissues.
Involvement in disease
Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
Post-translational
modifications
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.
Cellular localization
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionP10415
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
Database links
- Entrez Gene: 281020 Cow
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Entrez Gene: 24224 Rat
- Omim: 151430 Human
- SwissProt: BCL2 Cow
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
see all
Anti-Bcl2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
Fixation: 4% paraformaldehyde, paraffin embedding. Before the beginning of the procedure, slides are put in a 200 ml Coplin jar filled with 10 mM citrate buffer and heated in a commercial microwave oven operating at a frequency of 2.45 GHz and 600 W power setting. After two heating cycles of 5 minutes each, slides are allowed to cool at room temperature and thoroughly washed in phosphate buffered saline (PBS) pH 7.4 0.1 M. ABC peroxidase + Nickel intensification. Conterstaining Methyl green
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
Sample treated using same protocol described in the above image.
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Immunohistochemistry (Paraffin-embedded sections) - Bcl2 antibody (ab7973)
Sample treated using same protocol described in the above image, except with no countertaining.
Prof. Adalberto Merighi, Veterinary School, Univ. of Torino, Italy
Western blot - Bcl2 antibody (ab7973)
Anti-Bcl2 antibody (ab7973) at 1/500 dilution + 25 ug HeLa whole cell lysate
Secondary
Goat anti rabbit HRP at 1/4000 dilution
This image is an edited version of an image submitted courtesy of an anonymous Abreview on 30 September 2005. We do not have any further information relating to this image.
Immunocytochemistry/ Immunofluorescence-Bcl2 antibody(ab7973)
ICC/IF image of ab7973 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Bcl2 antibody (ab7973)
This product has been referenced in:
- Kelsen Set al. Endothelin-A receptor blockade slows the progression of renal injury in experimental renovascular disease. Am J Physiol Renal Physiol 301:F218-25 (2011).Read more (PubMed: 21478482) »
- Salvetti NRet al. Cell proliferation and survival mechanisms underlying the abnormal persistence of follicular cysts in bovines with cystic ovarian disease induced by ACTH. Anim Reprod Sci 122:98-110 (2010). IHC-P; Cow.Read more (PubMed: 20800980) »
See all 11 publications for this product
Publishing research using ab7973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"