Bcl2 protein (His tag) (ab79942)
Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Our Abpromise guarantee covers the use of ab79942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab79942 is useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Apoptosis regulator Bcl 2Apoptosis regulator Bcl-2Apoptosis regulator Bcl2
- AW986256B cell CLL/lymphoma 2B cell leukemia/lymphoma 2Bcl-2Bcl2BCL2_HUMANC430015F12RikD630044D05RikD830018M01RikLeukemia/lymphoma, B-cell, 2Oncogene B-cell leukemia 2PPP1R50Protein phosphatase 1, regulatory subunit 50
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.
References for Bcl2 protein (His tag) (ab79942)
ab79942 has not yet been referenced specifically in any publications.