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Overview

  • Product nameBcl2 protein (His tag)See all Bcl2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-211 of Human Bcl2 with N terminal His tag; 231 amino acids, 25.4 kDa. Swiss ID = P10415.
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab84209 is purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 2mM DTT, pH 8.0
  • Concentration information loading...
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84209 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab7973,ab15182,ab15183,ab16779,ab18210,ab37899,ab79204,ab117115,ab119113,.

    • Protein info

    • Alternative names
        Apoptosis regulator Bcl 2Apoptosis regulator Bcl-2Apoptosis regulator Bcl2
        AW986256B cell CLL/lymphoma 2B cell leukemia/lymphoma 2Bcl-2Bcl2BCL2_HUMANC430015F12RikD630044D05RikD830018M01RikLeukemia/lymphoma, B-cell, 2Oncogene B-cell leukemia 2PPP1R50Protein phosphatase 1, regulatory subunit 50
      see all
  • FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
  • Tissue specificityExpressed in a variety of tissues.
  • Involvement in diseaseNote=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
  • Post-translational
    modifications    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability.
  • Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.

    • Target information above from: UniProt accession P10415 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Bcl2 protein (His tag) images

    • SDS-PAGE - Bcl2 protein (His tag) (ab84209)
      SDS-PAGE - Bcl2 protein (His tag) (ab84209)
      15% SDS-PAGE showing ab84209 at approximately 25.4kDa (3µg).

    References for Bcl2 protein (His tag) (ab84209)

    ab84209 has not yet been referenced specifically in any publications.

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