Bcl2L2 protein (His tag) (ab58944)

Product Name 

Bcl2L2 protein (His tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant fragment, corresponding to amino acids 1-172 of Human Bcl2L2 (minus the 17 amino acid C-terminus, the mitochondrial targeting sequence), and with a histidine tag on the C-terminus. M.Wt 18 kDa (monomer), ~25% exists as a disulfide-linked dimer.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.

This product migrates as a polypeptide of 18 kDa on SDS-PAGE under both reducing and non-reducing conditions. A band corresponding to a disulfide-linked dimer is observed with non-reducing PAGE.

Biological Activity: This protein was assayed for its ability to inhibit the release of cytochrome c from isolated mouse liver mitochondria induced by caspase-8 cleaved human BID using a mouse/rat cytochrome c ELISA assay.
The EC₅₀ for human Bcl2L2 in this assay ranges from 50 to 250nM in the presence of 54 nM of caspase 8-cleaved human BID.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

Relevance 

Bcl 2L2 or Bcl-w is a member of the Bcl 2 family of proteins that regulates outer mitochondrial membrane permeability. At least fifteen Bcl 2 family members have been identified in mammalian cells. Pro-survival members of the Bcl 2 family, which include Bcl XL, Bcl X, Mcl 1 and A1, inhibit apoptosis in response to a wide variety of cytotoxic insults, whereas the proapoptotic family members (e.g. Bax, Bak, Bad, Bik and Bid) in general antagonize the function of the prosurvival family members. Bcl w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl w contains a carboxylterminal mitochondria targeting sequence. Recombinant Bcl w lacks the mitochondrial targeting sequence but maintains its ability to neutralize pro-apoptotic Bcl 2 family members. Neutralization by Bcl w appears to occur through binding the BH3 region of pro-apoptotic Bcl 2 family members. This activity does not require the mitochondrial targeting sequence.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 10% Glycerol, 0.1M Potassium chloride, 25mM HEPES, pH 7.5

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

This protein was expressed in Escherichia coli then purified from the soluble fraction of disrupted cells. Detergents were not used in the purification process.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

No change in EC₅₀ or dimerization has been observed in recombinant human Bcl2L2 that is stored at -20 °C.

Expression system 

E. coli

Sequence 

Amino acid sequencing indicates that the N-terminal methionine is
removed during expression in Escherichia coli.

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