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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Overview
Product name
Bcl2L2 protein (His tag)
See all Bcl2L2 products (2) ...
Protein description
Recombinant fragment, corresponding to amino acids 1-172 of Human Bcl2L2 (minus the 17 amino acid C-terminus, the mitochondrial targeting sequence), and with a histidine tag on the C-terminus. M.Wt 18 kDa (monomer), ~25% exists as a disulfide-linked dimer.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
This protein was expressed in Escherichia coli then purified from the soluble fraction of disrupted cells. Detergents were not used in the purification process.
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 0.1M Potassium chloride, 25mM HEPES, pH 7.5
Concentration
Concentration information loading...
Additional notes
No change in EC50 or dimerization has been observed in recombinant human Bcl2L2 that is stored at -20 °C.
Sequence notes
Amino acid sequencing indicates that the N-terminal methionine is remove d during expression in Escherichi a coli.
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Cell survival & death
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
This product migrates as a polypeptide of 18 kDa on SDS-PAGE under both reducing and non-reducing conditions. A band corresponding to a disulfide-linked dimer is observed with non-reducing PAGE.
Biological Activity: This protein was assayed for its ability to inhibit the release of cytochrome c from isolated mouse liver mitochondria induced by caspase-8 cleaved human BID using a mouse/rat cytochrome c ELISA assay.
The EC50 for human Bcl2L2 in this assay ranges from 50 to 250nM in the presence of 54 nM of caspase 8-cleaved human BID.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
Tissue specificity
Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
The BH4 motif seems to be involved in the anti-apoptotic function.
The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
Cellular localization
Mitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
Target information above from: UniProt accessionQ92843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Bcl2L2 protein (His tag) (ab58944)
ab58944 has not yet been referenced specifically in any publications.
Publishing research using ab58944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"