Anti-Bcl3 antibody [Ham150-3.5] (ab15046)
Key features and details
- Hamster monoclonal [Ham150-3.5] to Bcl3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl3 antibody [Ham150-3.5]
See all Bcl3 primary antibodies -
Description
Hamster monoclonal [Ham150-3.5] to Bcl3 -
Host species
Hamster -
Specificity
ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms. -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length protein corresponding to Mouse Bcl3.
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Positive control
- C57BI/6 human T cells and Bcl3 transgenic ms T cells. Flow Cyt: BAF3, Ramos cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Ham150-3.5 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
1 µg/test (100µl) |
Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa). |
Flow Cyt
Use at an assay dependent concentration. 1 µg/test (100µl) |
Target
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Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
Sequence similarities
Contains 7 ANK repeats. -
Post-translational
modificationsPolyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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Database links
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
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Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Images
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Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Flow cytometric analysis of 2% paraformaldehyde-fixed BAF3cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/ml
Predicted band size: 50 kDa
Western blot of Bcl3 in transgenic mouse T cells using Ab15046.
Datasheets and documents
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Datasheet download
References (0)
ab15046 has not yet been referenced specifically in any publications.