![Immunohistochemistry (Paraffin-embedded sections) - Bcl3 antibody [1E8] (ab49470) image](#ab49470_1.jpg)
 image](#Bcl3-Primary-antibodies-ab49470-1.jpg)
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Products:Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Overview
Product name
Anti-Bcl3 antibody [1E8]
See all Bcl3 products (5) ...
Description
Mouse monoclonal [1E8] to Bcl3
Tested applications
ICC/IF, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant protein corresponding to a 200 amino acid N terminal truncate of the Bcl-3 molecule.
Positive control
Immunohistochemistry: Tonsil.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
1E8
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/25 - 1/50.
IHC-Fr: 1/100(Acetone fixation is recommended.)
Application notes
Is unsuitable for or WB.
Target
Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
Sequence similarities
Contains 7 ANK repeats.
Post-translational
modifications
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
Target information above from: UniProt accessionP20749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Database links
- Entrez Gene: 602 Human
- Omim: 109560 Human
- SwissProt: P20749 Human
- Unigene: 31210 Human
Anti-Bcl3 antibody [1E8] images:
Immunohistochemistry (Paraffin-embedded sections) - Bcl3 antibody [1E8] (ab49470)
![Immunohistochemistry (Paraffin-embedded sections) - Bcl3 antibody [1E8] (ab49470)](/ps/datasheet/Images/49/ab49470/ab49470_1.jpg)
ab49470, at a dilution of 1/25, staining Bcl3 oncoprotein in paraffin sections of normal human tonsil by immunohistochemistry. Note nuclear staining of a proportion of follicular cells, parafollicular cells and mucosa.
Immunocytochemistry/ Immunofluorescence-Bcl3 antibody [1E8](ab49470)
](/ps/datasheet/images/49/ab49470/Bcl3-Primary-antibodies-ab49470-1.jpg)
ICC/IF image of ab49470 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49470, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Bcl3 antibody [1E8] (ab49470)
ab49470 has not yet been referenced specifically in any publications.
Publishing research using ab49470? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"