![Western blot - Bcl3 antibody [Ham150-3.5] (ab15046) image](#various.jpg)
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Products:Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Overview
Product name
Anti-Bcl3 antibody [Ham150-3.5]
See all Bcl3 products (5) ...
Description
Hamster monoclonal [Ham150-3.5] to Bcl3
Specificity
Ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Full length mouse Bcl3 protein.
Positive control
C57BI/6 human T cells and Bcl3 transgenic ms T cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
Ham150-3.5
Isotype
IgG
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Western blot - Bcl3 antibody [Ham150-3.5] (ab15046)
![Western blot - Bcl3 antibody [Ham150-3.5] (ab15046) image](/ps/datasheet/Images/15/ab15046/various.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 2 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 50 kDa).
Target
Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
Sequence similarities
Contains 7 ANK repeats.
Post-translational
modifications
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
Target information above from: UniProt accessionP20749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Database links
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q3U300 Mouse
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
Anti-Bcl3 antibody [Ham150-3.5] images:
Western blot - Bcl3 antibody [Ham150-3.5] (ab15046)
Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/ml
Predicted band size : 50 kDa
References for Anti-Bcl3 antibody [Ham150-3.5] (ab15046)
ab15046 has not yet been referenced specifically in any publications.
Publishing research using ab15046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"