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Products:Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Overview
Product name
Anti-Bcl3 antibody
See all Bcl3 products (5) ...
Description
Rabbit polyclonal to Bcl3
Tested applications
WB, IP, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) - mapping to the C terminus of Bcl 3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1.0% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Bcl3 antibody (ab27780)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 4 µg/ml.Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
IP: Use a concentration of 5 - 10 µg/ml.
IHC-Fr: Use a concentration of 20 - 40 µg/ml.
Target
Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
Sequence similarities
Contains 7 ANK repeats.
Post-translational
modifications
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
Target information above from: UniProt accessionP20749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
Database links
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Entrez Gene: 680611 Rat
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q3U300 Mouse
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
Anti-Bcl3 antibody images:
Western blot - Bcl3 antibody (ab27780)
Anti-Bcl3 antibody (ab27780) at 2 µg/ml + Jurkat
Predicted band size : 47 kDa
References for Anti-Bcl3 antibody (ab27780)
ab27780 has not yet been referenced specifically in any publications.
Publishing research using ab27780? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"